The secondary antibody is usually linked to biotin or to a reporter enzyme such as alkaline phosphatase or horseradish peroxidase.

Howard loves the Yiddish expression To a worm in horseradish, the world is horseradish.

This was his horseradish. He was obsessed with it!

So far, eight different isoforms of glutathione peroxidase (GPx1 8) have been identified in humans.

Myeloperoxidase (MPO) is a peroxidase enzyme that in humans is encoded by the MPO gene.

Glutathione peroxidase 1 (GPx1) is the most abundant version, found in the cytoplasm of nearly all mammalian tissues, whose preferred substrate is hydrogen peroxide.

Colorimetric detection The colorimetric detection method depends on incubation of the western blot with a substrate that reacts with the reporter enzyme (such as peroxidase) that is bound to the secondary antibody.